Metabolic Pathway of 2-Deoxy-D-Ribose

Metabolic pathway of 2-Deoxy-D-Ribose, a deoxy sugar derived from ribose, primarily involves its catabolism and utilization in nucleotide synthesis. It plays a crucial role as a component of deoxyribonucleotides (dNTPs), which are essential for DNA synthesis and repair.

Metabolic Pathway of 2-Deoxy-D-Ribose:

1. Phosphorylation of 2-Deoxy-D-Ribose

  • 2-Deoxy-D-Ribose is phosphorylated by the enzyme deoxyribokinase (EC 2.7.1.110) to form 2-Deoxy-D-Ribose-5-Phosphate.
  • Reaction:

2-Deoxy-D-Ribose+ATP→2-Deoxy-D-Ribose-5-Phosphate+ADP

2. Cleavage via the Pentose Phosphate Pathway (PPP)

  • Deoxy-D-Ribose-5-Phosphate undergoes cleavage by phosphodeoxyriboaldolase (EC 4.1.2.4) into:
  • D-glyceraldehyde-3-phosphate (G3P) (enters glycolysis)
  • Acetaldehyde (converted into acetate or ethanol in certain organisms)

Reaction:

2-Deoxy-D-Ribose-5-Phosphate→D-Glyceraldehyde-3-Phosphate+Acetaldehyde

Metabolic Pathway of 2-Deoxy-D-Ribose-Xi'an Lyphar Biotech Co., Ltd

3. Entry into Central Metabolism

    Glyceraldehyde-3-Phosphate enters glycolysis, leading to ATP production.

    Acetaldehyde can be further metabolized to:

    • Acetate (via aldehyde dehydrogenase) for energy generation.
    • Ethanol (via alcohol dehydrogenase in anaerobic organisms).

    4. Alternative Pathway: Nucleotide Synthesis

    • 2-Deoxy-D-Ribose can also be incorporated into nucleotide synthesis via salvage pathways.
    • Deoxyribonucleotide synthesis: It contributes to deoxyribose moieties in deoxyribonucleotides (dNTPs) used for DNA replication and repair.

    Key Enzymes Involved:

    • Deoxyribokinase (EC 2.7.1.110) – phosphorylates 2-Deoxy-D-Ribose.
    • Phosphodeoxyriboaldolase (EC 4.1.2.4) – cleaves Deoxy-Ribose-5-Phosphate into glycolytic intermediates.
    • Aldehyde dehydrogenase (EC 1.2.1.3) – oxidizes acetaldehyde to acetate.
    • Alcohol dehydrogenase (EC 1.1.1.1) – converts acetaldehyde to ethanol (in fermentative organisms).
    Metabolic Pathway of 2-Deoxy-D-Ribose-Xi'an Lyphar Biotech Co., Ltd

    Biological Significance

    • DNA metabolism: As a precursor for deoxyribonucleotides.
    • Energy production: Via glycolytic and oxidative pathways.
    • Salvage Pathway: Helps in nucleotide recycling, conserving metabolic energy.

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