Materials and Methods of 5-Amino-1MQ Iodide

5-Amino-1MQ Iodide (5-Amino-1-methylquinolinium iodide), also known as 5-Amino-1MQ, is a small molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme implicated in various metabolic processes.

Materials of 5-Amino-1MQ Iodide

Chemicals & Reagents

1. 5-Amino-1MQ Iodide (5-Amino-1-methylquinolinium iodide) was synthesized following standard methylation and iodination protocols.

2. Methylating agent: Methyl iodide (CH₃I) (Sigma-Aldrich, 99%)

3. Base: Potassium carbonate (K₂CO₃) (Sigma-Aldrich, 99%)

4. Solvents:

    • Acetone (anhydrous, 99%)
    • Ethanol (absolute, 99.9%)
    • Diethyl ether (99%)

    5. Deionized (DI) water

      Materials and Methods of 5-Amino-1MQ Iodide-Xi'an Lyphar Biotech Co., Ltd

      Equipment

      • Magnetic stirrer with heating
      • Reflux apparatus
      • Rotary evaporator
      • Buchner funnel with vacuum filtration
      • NMR spectrometer (400 MHz)
      • IR spectrometer
      • Melting point apparatus

      Methods of 5-Amino-1MQ Iodide

      Synthesis of 5-Amino-1MQ Iodide (5-Amino-1-methylquinolinium iodide)

      1. Methylation of 5-Aminoquinoline

        • 5-Aminoquinoline (1.0 mmol) was dissolved in acetone (10 mL) under stirring.
        • Potassium carbonate (1.2 mmol) was added to the solution to act as a base.
        • Methyl iodide (1.1 mmol) was added dropwise to the reaction mixture at room temperature.
        • The reaction was stirred at 50°C for 6 hours under reflux.

        2. Isolation of the Product

          • The reaction mixture was cooled to room temperature and the solvent was evaporated under reduced pressure.
          • The residue was dissolved in a minimal amount of ethanol and precipitated by adding diethyl ether.
          • The solid was collected by vacuum filtration and washed with cold ethanol.
          • The crude product was dried under vacuum at 50°C for 12 hours.
          Materials and Methods of 5-Amino-1MQ Iodide-Xi'an Lyphar Biotech Co., Ltd

          3. Purification

            • The solid product was recrystallized from ethanol and dried to obtain pure 5-Amino-1MQ Iodide .

            4. Characterization

              • NMR Spectroscopy (¹H and ¹³C NMR): Confirmed structure and purity.
              • Infrared Spectroscopy (IR): Verified functional groups.
              • Melting Point Analysis: Determined the purity and thermal stability of the compound.

              This protocol provides a straightforward approach to synthesizing 5-Amino-1MQ Iodide with high purity. Let me know if you need modifications based on your specific setup!