Biotinoyl Tripeptide-1 is a synthetic peptide widely used in hair and lash care formulations. It combines a vitamin moiety with a short peptide chain to enhance biological activity and stability.
Chemical Structure of Biotinoyl Tripeptide-1
Basic Composition
Biotinoyl Tripeptide-1 is composed of:
Biotin (Vitamin B7) – a lipophilic vitamin
Tripeptide-1 sequence – typically:
- Glycine (Gly)
- Histidine (His)
- Lysine (Lys)
Full structure: Biotin–Gly–His–Lys (Biotinyl-GHK)
Structural Features
Peptide bonds link the three amino acids (–CO–NH–)
Amide linkage connects biotin to the N-terminus of the peptide
Contains:
- Amine groups (–NH₂)
- Carboxyl groups (–COOH)
- Imidazole ring (from histidine)
- Long valeric acid side chain (from biotin)
Molecular Information
- INCI Name: Biotinoyl Tripeptide-1
- Molecular Formula: ~C₂₄H₃₈N₈O₆S
- Molecular Weight: ~566–575 Da (varies slightly by salt form)
Physical Properties of Biotinoyl Tripeptide-1
Appearance
- White to off-white powder
- Sometimes supplied as a clear aqueous solution
Solubility
Water-soluble (due to peptide portion)
Slight amphiphilic character:
- Hydrophilic peptide
- Lipophilic biotin moiety
This dual nature improves skin and follicle penetration
Stability
Stable under:
- Neutral to slightly acidic pH (≈ 4.0–7.0)
Sensitive to:
- Strong acids/bases
- High temperatures
- Enzymatic degradation (proteases)
pH Range (Formulation)
- Optimal: pH 4.5 – 6.5
Storage Conditions
Store:
- In a cool, dry place
- Protected from light
- Often refrigerated for long-term stability
Functional Physicochemical Characteristics
Molecular Size
- Small peptide → good bioavailability in topical applications
Charge Properties
Zwitterionic behavior depending on pH:
- Histidine can be protonated/deprotonated
- Lysine is positively charged at physiological pH
Hygroscopicity
- Slightly hygroscopic (absorbs moisture)
Cosmetic-Relevant Properties
Compatible with:
- Water-based serums
- Emulsions
- Hair tonics
Often used at:
- 0.001% – 0.1% concentration
Summary
Biotinoyl Tripeptide-1 is a biotinylated signaling peptide (GHK) with:
- A hybrid structure (vitamin + peptide)
- Good water solubility and stability in mild conditions
- Favorable skin/hair penetration characteristics
- Functional groups enabling biological activity and formulation flexibility
If you want, I can also provide:
- Structural diagram (2D/3D visualization)
- Synthesis pathway (solid-phase peptide synthesis)
- Spectral data (MS, HPLC, NMR)