The Application of Bacillus pumilus

Bacillus pumilus belongs to the bacterial kingdom, hard minutes, Bacillales, Bacillaceae, Bacillus genus. The bacterial body is rod-shaped, round end, single or short chain arrangement, the bacterial body size 1.2~1.5um×2.0~4.0um, motility, Gram-positive, spore oval, intermediate or secondary, size 1.0~1.2um×1.5~2.0um. Can hydrolyze starch, degrade mannan, xylan, cellulose, etc. Its working mechanism is: to produce a variety of exogenous digestive enzymes, such as xylanase, cellulase, and biological oxygen, promote the growth and reproduction of beneficial anaerobic microorganisms, maintain the intestinal ecological balance; 

Bacillus pumilus is one of the important PGPR bacteria in the soil and plant rhizome can produce peptides, proteins, pyrazines, phenols, and other antibacterial substances, and has the dual effect of disease prevention and growth promotion. Bacillus brevis is also a common microorganism in some humic acid feedstocks. It can not only induce plant disease resistance but also can colonize the plant root tip and form a biofilm, promoting the plant’s absorption of nutrients. B. brevis can survive in the rhizosphere soil of rice seedlings for over 15d.

The role of Bacillus pumilus in agriculture

1. Bacillus pumilus can enhance soil nutrients, significantly improve the degradation rate of plant straw, and can increase the content of quick nitrogen and quick phosphorus in the soil.

2. Bacillus pumilus can promote plant growth, especially in Arabidopsis, tobacco, soybean, and hot pepper. It is manifested in promoting seed germination, promoting the root system, bud, and seedling growth.

3. Bacillus pumilus fungicide can also enhance plant disease resistance and reduce the incidence of bacterial, fungal, and viral diseases, thus increasing production to 10 to 30%. It can induce the disease resistance of tobacco and cucumber plants to systemic viral diseases and bacterial leaf spot disease.

4. Bacillus pumilus fungicide can significantly reduce the heavy metal content in plants. Bacillus pumilus absorbs cadmium in the soil, thus reducing the absorption of cadmium in rice roots and reducing the solid cadmium concentration in rice seeds by 10%.

5. Bacillus pumilus fungicide also exhibits significant deodorization effects.

6. Bacillus pumilus can be used as a saline soil improvement species. Under salt stress inoculation of Bacillus pumilus short can not only promote the growth of licorice, and alleviate the damage of salt stress to licorice, but also can improve the antioxidant enzyme activity of soybean, wheat, tomato, corn, and other crops, thus enhancing the ability of crops to remove reactive oxygen species, enhance the salt resistance of crops, to promote the growth and development of plants.

Application of Bacillus pumilus in feed

1. Bacillus pumilus can significantly reduce the ratio of meat in the later stage of broiler breeding, and the depth of the duodenal crypts is significantly different from the control and can make the crypts of each segment of the intestinal canal shorter, which has advantages in the increasing survival rate of epithelial cells.

2. The metabolites of Bacillus pumilus mainly include auxin, antimicrobial peptides, amino acids, etc., among which the most obvious role of auxin is to promote growth. Antimicrobial peptides have the biological activity to inhibit fungi such as mold, Gram-positive bacteria, Gram-negative bacteria, and some viruses, and are widely used in feed additives and other fields because of their broad-spectrum antibacterial capabilities.

2. Bacillus pumilus can replace adriamycin as a growth-promoting agent in the feed.

3. Bacillus pumilus can also degrade non-starch polysaccharides, improve feed utilization rate, promote the digestion and absorption of nutrients in feed; promote animal intestinal development, enhance the activity of endogenous digestive enzymes, improve daily weight gain, reduce the ratio of meat.

4. Bacillus pumilus can also enhance the immune function of animals and improve their ability to resist stress.

5. Bacillus pumilus can produce a variety of exogenous digestive enzymes, such as xylanase and cellulase.

6. Bacillus pumilus can decompose feces, have less substrate pollution, and reduce the generation of ammonia nitrogen, hydrogen sulfide, and other harmful substances.

Usage and dosage

Take 10 billion / g as an example: by 100-150g / ton in the feed granulation added or mixing material, drinking water use.

Matters that need attention:

1. Before strain activation, keep the cryotube in a low-temperature, clean and dry environment, and placing it at room temperature for a long time will lead to strain decline;

2. Opening of frozen tubes, redissolution of lyophilized powder, and recovery of strains should be carried out under sterile conditions;

3. After some strains are frozen and dried, the delay period is long, and some need to be cultured in two consecutive times before normal growth;

4. The culture of caustic trophic bacteria should be made in a culture medium containing specific nutrients. Please choose it correctly and call me when it is not clear;

5. The culture of some anaerobic bacteria shall be filled with anaerobic gas from the opening to the inoculation to maintain the anaerobic state, and also maintain the anaerobic state during the culture process;

6. Some strains, such as Streptococcus pneumonia, Haemophilus influenza, and Neisseria gonorrhoeae, need 5-10%CO2 to promote growth;

7. If abnormal conditions such as the loose frozen tube cover and the cloudy compound solution are found, the corresponding products should be stopped.

8. Some bacteria species are pathogenic and spreading, and professionals are requested to have protective operations in a professional environment.

Application of Bacillus pumilus in medical treatment

Bacillus pumilus also has a certain role in medical treatment, treatment of burn Bacillus pumilus, fermentation broth by centrifugation supernatant, not only can significantly inhibit the burn wound infection caused by Staphylococcus aureus and Pseudomonas aeruginosa growth, and can spray on burn wound achieve the effect of preventing infection and promoting wound healing.